Method Used: Haemiglobincyanide (HiCN) Method.
Principle: whole blood is diluted 1 in 201 dilutions in a modified Drabkin’s solution which contains potassium cyanide. The red cells are haemolyzed and the Hb is oxidized by the ferricyanide to methaemoglobin. This is converted by the cyanide to stable hemoglobin cyanide (HiCN). Absorbance of the HiCN solution is read in a spectrophotometer at wavelength 540nm or in a filter Colorimeter using a yellow-green filter (Cheesbrough, 2000) .
Procedure:
1. measured 20µ1 (0.02ml) of capillary blood on well –mixed venous blood and dispense it into 4ml Drabkin’s neutal diluting fluid
2. stoppered the tube, mixed and left the diluted blood at room temperature protected from sunlight for 5-5minutes
3. placed the yellow –green filter in a colorimeter or set at a wavelength at 540nm
4. zero the colorimeter with Drabkins fluid and read the absorbance of the patients sample (CHEESBROUGHT, 2000)
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