Blood film making is of great importance to haematology because it forms a daily routine tool of diagnosis in haematology laboratory. Blood film is significant in:
MICROSCOPIC EXAMINATIONS FOR DIAGNOSTIC CELL COUNTS SUCH AS:
· Rough estimation of cell counts:
Under the low power (10x) objective, the RBC’s appear as small, round, reddish orange bodies and are the major cell types. Scattered among the RBC’s are the white cells. The white cells are nucleated and their nuclei stain in shades of purple and the cytoplasm’s stain in different colours, depending on the type of cell. In an area where the cells are evenly spread in a single layer, 5 white blood cells in low a power field are roughly equal to 1000 red cells / cu.mm (1×109 cells /L). At least 5 low power fields should be counted to average the estimate of white cell count.
· Evaluation of platelet count
· White blood cell count (Ochei and Kolhatkar, 2007).
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