Biofilm Production and Antibiotic Susceptibility of Uropathogens Isolated from Ahmadu Bello University Teaching Hospital Zaria, Nigeria

 – Biofilm Production and Antibiotic Susceptibility of Uropathogens Isolated from Ahmadu Bello University Teaching Hospital Zaria, Nigeria –

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ABSTRACT

on surfaces (animate and inanimate object) by microorganisms has become a major concern now in medical settings since it poses threat of induction and

Uropathogens as associated with catheterization, ureter and bladder surfaces is of a major interest as far as biofilm production is concerned.

This study determined the biofilm production and antibiotic susceptibility pattern of uropathogens isolated from Ahmadu Bello University Teaching Hospital, Zaria.

Isolates from urine samples submitted to the Medical Microbiology Laboratory of the hospital were collected over a period of five months and identified using standard microbiological techniques.

Alongside, a three months retrospective study within the period of the sample collections was carried out to give a prevalent rate of the occurrence of urinary tract infections in the study area.

Further more, the isolates were evaluated for their biofilm production potentials and their susceptibility to antibiotics were determined.

The Cefoxitin Disc Test for MRSA and the D-test for erythromycin-resistant S. aureus were also carried out. From the retrospective study, Klebsiella spp were the most common bacteria isolated (36.40%), followed by E. coli (34.98%), S. aureus (7.77%), Proteus spp (2.47%), Pseudomonas spp (1.77%) and others including:

INTRODUCTION

1.1 Background of the Study

Several pathogens associated with have been linked to chronic infections and diseases as a result of biofilm production.

This has been seen of P. aeruginosa in cystic fibrosis, S. pneumonia in chronic otitis media, S. aureus in chronic rhinosinusitis and enteropathogenic E. coli in recurrent urinary tract infections (Hall- Stoodley and Stoodley, 2009).

The in the management of urinary tract infections is a serious public health issue, particularly in the developing world where apart from high level of poverty, ignorance and poor hygienic practices, there is also high prevalence of fake drugs of questionable quality in circulation (El- Astal, 2005).

This among uropathogens is said to have increased over the past 30 years (Zhanel, 2000).

The resistance is mostly encountered with the antibiotics frequently used for the treatment of these infections and it varies among communities depending on their prescription pattern.

The worldwide data generally, is showing an increasing resistance among the uropathogens (Hryniewicz et al., 2001).

These problematic uropathogens include: E. coli, Klebsiella spp, Enterobacter spp, Citrobacter spp, Proteus spp, Providencia stuart, Ps. aeruginosa, Staphylococcus spp, etc.

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